Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.
Identifieur interne : 000070 ( PubMed/Corpus ); précédent : 000069; suivant : 000071Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.
Auteurs : Hinda Abib ; Fatima Laraba-DjebariSource :
- Canadian journal of physiology and pharmacology [ 0008-4212 ] ; 2003.
English descriptors
- KwdEn :
- Animals, Cobalt Radioisotopes, Creatine Kinase (analysis), Creatine Kinase (blood), Edema (chemically induced), Edema (pathology), Foot, Gamma Rays, Hemorrhage (chemically induced), Hemorrhage (pathology), Immunization, Injections, Intradermal, Injections, Intraperitoneal, Lethal Dose 50, Male, Mice, Mice, Inbred Strains, Myocardium (enzymology), Myocardium (pathology), Necrosis, Skin (drug effects), Skin Diseases (chemically induced), Toxicity Tests, Acute, Viper Venoms (administration & dosage), Viper Venoms (immunology), Viper Venoms (radiation effects), Viper Venoms (toxicity), Viperidae.
- MESH :
- chemical , administration & dosage : Viper Venoms.
- chemical , analysis : Creatine Kinase.
- chemical , blood : Creatine Kinase.
- chemical , immunology : Viper Venoms.
- chemical , radiation effects : Viper Venoms.
- chemical , toxicity : Viper Venoms.
- chemical : Cobalt Radioisotopes.
- chemically induced : Edema, Hemorrhage, Skin Diseases.
- drug effects : Skin.
- enzymology : Myocardium.
- pathology : Edema, Hemorrhage, Myocardium.
- Animals, Foot, Gamma Rays, Immunization, Injections, Intradermal, Injections, Intraperitoneal, Lethal Dose 50, Male, Mice, Mice, Inbred Strains, Necrosis, Toxicity Tests, Acute, Viperidae.
Abstract
Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals.
DOI: 10.1139/y03-121
PubMed: 14719031
Links to Exploration step
pubmed:14719031Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.</title><author><name sortKey="Abib, Hinda" sort="Abib, Hinda" uniqKey="Abib H" first="Hinda" last="Abib">Hinda Abib</name><affiliation><nlm:affiliation>Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne Bab Ezzouar, Alger, Algérie.</nlm:affiliation></affiliation></author><author><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14719031</idno><idno type="pmid">14719031</idno><idno type="doi">10.1139/y03-121</idno><idno type="wicri:Area/PubMed/Corpus">000070</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000070</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.</title><author><name sortKey="Abib, Hinda" sort="Abib, Hinda" uniqKey="Abib H" first="Hinda" last="Abib">Hinda Abib</name><affiliation><nlm:affiliation>Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne Bab Ezzouar, Alger, Algérie.</nlm:affiliation></affiliation></author><author><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name></author></analytic><series><title level="j">Canadian journal of physiology and pharmacology</title><idno type="ISSN">0008-4212</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cobalt Radioisotopes</term><term>Creatine Kinase (analysis)</term><term>Creatine Kinase (blood)</term><term>Edema (chemically induced)</term><term>Edema (pathology)</term><term>Foot</term><term>Gamma Rays</term><term>Hemorrhage (chemically induced)</term><term>Hemorrhage (pathology)</term><term>Immunization</term><term>Injections, Intradermal</term><term>Injections, Intraperitoneal</term><term>Lethal Dose 50</term><term>Male</term><term>Mice</term><term>Mice, Inbred Strains</term><term>Myocardium (enzymology)</term><term>Myocardium (pathology)</term><term>Necrosis</term><term>Skin (drug effects)</term><term>Skin Diseases (chemically induced)</term><term>Toxicity Tests, Acute</term><term>Viper Venoms (administration & dosage)</term><term>Viper Venoms (immunology)</term><term>Viper Venoms (radiation effects)</term><term>Viper Venoms (toxicity)</term><term>Viperidae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Creatine Kinase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Creatine Kinase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="radiation effects" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cobalt Radioisotopes</term></keywords><keywords scheme="MESH" qualifier="chemically induced" xml:lang="en"><term>Edema</term><term>Hemorrhage</term><term>Skin Diseases</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Skin</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Myocardium</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Edema</term><term>Hemorrhage</term><term>Myocardium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Foot</term><term>Gamma Rays</term><term>Immunization</term><term>Injections, Intradermal</term><term>Injections, Intraperitoneal</term><term>Lethal Dose 50</term><term>Male</term><term>Mice</term><term>Mice, Inbred Strains</term><term>Necrosis</term><term>Toxicity Tests, Acute</term><term>Viperidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14719031</PMID><DateCreated><Year>2004</Year><Month>1</Month><Day>13</Day></DateCreated><DateCompleted><Year>2004</Year><Month>08</Month><Day>24</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0008-4212</ISSN><JournalIssue CitedMedium="Print"><Volume>81</Volume><Issue>12</Issue><PubDate><Year>2003</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Canadian journal of physiology and pharmacology</Title><ISOAbbreviation>Can. J. Physiol. Pharmacol.</ISOAbbreviation></Journal><ArticleTitle>Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.</ArticleTitle><Pagination><MedlinePgn>1125-30</MedlinePgn></Pagination><Abstract><AbstractText>Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abib</LastName><ForeName>Hinda</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne Bab Ezzouar, Alger, Algérie.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laraba-Djebari</LastName><ForeName>Fatima</ForeName><Initials>F</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Can J Physiol Pharmacol</MedlineTA><NlmUniqueID>0372712</NlmUniqueID><ISSNLinking>0008-4212</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003037">Cobalt Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014757">Viper Venoms</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.3.2</RegistryNumber><NameOfSubstance UI="D003402">Creatine Kinase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003037" MajorTopicYN="N">Cobalt Radioisotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003402" MajorTopicYN="N">Creatine Kinase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004487" MajorTopicYN="N">Edema</DescriptorName><QualifierName UI="Q000139" MajorTopicYN="Y">chemically induced</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005528" MajorTopicYN="N">Foot</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005720" MajorTopicYN="Y">Gamma Rays</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006470" MajorTopicYN="N">Hemorrhage</DescriptorName><QualifierName UI="Q000139" MajorTopicYN="Y">chemically induced</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007114" MajorTopicYN="N">Immunization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007271" MajorTopicYN="N">Injections, Intradermal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007274" MajorTopicYN="N">Injections, Intraperitoneal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007928" MajorTopicYN="N">Lethal Dose 50</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008815" MajorTopicYN="N">Mice, Inbred Strains</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009206" MajorTopicYN="N">Myocardium</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009336" MajorTopicYN="N">Necrosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012867" MajorTopicYN="N">Skin</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012871" MajorTopicYN="N">Skin Diseases</DescriptorName><QualifierName UI="Q000139" MajorTopicYN="N">chemically induced</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023382" MajorTopicYN="N">Toxicity Tests, Acute</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014757" MajorTopicYN="N">Viper Venoms</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017819" MajorTopicYN="N">Viperidae</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>1</Month><Day>14</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>8</Month><Day>25</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>1</Month><Day>14</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14719031</ArticleId><ArticleId IdType="doi">10.1139/y03-121</ArticleId><ArticleId IdType="pii">y03-121</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000070 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000070 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:14719031
|texte= Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:14719031" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a CobaltMaghrebV1
| This area was generated with Dilib version V0.6.32. |  |