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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cell-Autonomous Expression of Barley <italic>Mla1</italic> Confers Race-Specific Resistance to the Powdery Mildew Fungus via a <italic>Rar1</italic>-Independent Signaling Pathway</title><author><name sortKey="Zhou, Fasong" sort="Zhou, Fasong" uniqKey="Zhou F" first="Fasong" last="Zhou">Fasong Zhou</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Kurth, Joachim" sort="Kurth, Joachim" uniqKey="Kurth J" first="Joachim" last="Kurth">Joachim Kurth</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Wei, Fusheng" sort="Wei, Fusheng" uniqKey="Wei F" first="Fusheng" last="Wei">Fusheng Wei</name><affiliation><nlm:aff id="aff2">Interdepartmental Genetics Program and Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation></author><author><name sortKey="Elliott, Candace" sort="Elliott, Candace" uniqKey="Elliott C" first="Candace" last="Elliott">Candace Elliott</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Vale, Giampiero" sort="Vale, Giampiero" uniqKey="Vale G" first="Giampiero" last="Valè">Giampiero Valè</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Yahiaoui, Nabila" sort="Yahiaoui, Nabila" uniqKey="Yahiaoui N" first="Nabila" last="Yahiaoui">Nabila Yahiaoui</name><affiliation><nlm:aff id="aff3">Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><affiliation><nlm:aff id="aff3">Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Somerville, Shauna" sort="Somerville, Shauna" uniqKey="Somerville S" first="Shauna" last="Somerville">Shauna Somerville</name><affiliation><nlm:aff id="aff4">Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305</nlm:aff></affiliation></author><author><name sortKey="Wise, Roger" sort="Wise, Roger" uniqKey="Wise R" first="Roger" last="Wise">Roger Wise</name><affiliation><nlm:aff id="aff2">Interdepartmental Genetics Program and Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Corn Insects and Crop Genetics Research, United States Department of Agriculture, Agricultural Research Service, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11226189</idno><idno type="pmc">102246</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC102246</idno><idno type="RBID">PMC:102246</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000220</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000220</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cell-Autonomous Expression of Barley <italic>Mla1</italic> Confers Race-Specific Resistance to the Powdery Mildew Fungus via a <italic>Rar1</italic>-Independent Signaling Pathway</title><author><name sortKey="Zhou, Fasong" sort="Zhou, Fasong" uniqKey="Zhou F" first="Fasong" last="Zhou">Fasong Zhou</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Kurth, Joachim" sort="Kurth, Joachim" uniqKey="Kurth J" first="Joachim" last="Kurth">Joachim Kurth</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Wei, Fusheng" sort="Wei, Fusheng" uniqKey="Wei F" first="Fusheng" last="Wei">Fusheng Wei</name><affiliation><nlm:aff id="aff2">Interdepartmental Genetics Program and Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation></author><author><name sortKey="Elliott, Candace" sort="Elliott, Candace" uniqKey="Elliott C" first="Candace" last="Elliott">Candace Elliott</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Vale, Giampiero" sort="Vale, Giampiero" uniqKey="Vale G" first="Giampiero" last="Valè">Giampiero Valè</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author><author><name sortKey="Yahiaoui, Nabila" sort="Yahiaoui, Nabila" uniqKey="Yahiaoui N" first="Nabila" last="Yahiaoui">Nabila Yahiaoui</name><affiliation><nlm:aff id="aff3">Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><affiliation><nlm:aff id="aff3">Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Somerville, Shauna" sort="Somerville, Shauna" uniqKey="Somerville S" first="Shauna" last="Somerville">Shauna Somerville</name><affiliation><nlm:aff id="aff4">Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305</nlm:aff></affiliation></author><author><name sortKey="Wise, Roger" sort="Wise, Roger" uniqKey="Wise R" first="Roger" last="Wise">Roger Wise</name><affiliation><nlm:aff id="aff2">Interdepartmental Genetics Program and Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Corn Insects and Crop Genetics Research, United States Department of Agriculture, Agricultural Research Service, Iowa State University, Ames, Iowa 50011-1020</nlm:aff></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation><nlm:aff id="aff1">The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</nlm:aff></affiliation></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The barley <italic>Mla</italic> locus encodes 28 characterized resistance specificities to the biotrophic fungal pathogen barley powdery mildew. We describe a single-cell transient expression assay using entire cosmid DNAs to pinpoint <italic>Mla1</italic> within the complex 240-kb <italic>Mla</italic> locus. The MLA1 cDNA encodes a 108-kD protein containing an N-terminal coiled-coil structure, a central nucleotide binding domain, and a C-terminal leucine-rich repeat region; it also contains a second short open reading frame at the 5′ end that has a possible regulatory function. Although most <italic>Mla-</italic>encoded resistance specificities require <italic>Rar1</italic> for their function, we used the single-cell expression system to demonstrate that <italic>Mla1</italic> triggers full resistance in the presence of the severely defective <italic>rar1-2</italic> mutant allele. Wheat contains an ortholog of barley <italic>Mla</italic>, designated <italic>TaMla</italic>, that is tightly linked to (0.7 centimorgan) but distinct from a tested resistance specificity at the complex <italic>Pm3</italic> locus to wheat powdery mildew. Thus, the most polymorphic powdery mildew resistance loci in barley and wheat may have evolved in parallel at two closely linked homeoloci. Barley <italic>Mla1</italic> expressed in wheat using the single-cell transformation system failed to trigger a response to any of the wheat powdery mildew <italic>Avr</italic> genes tested, indicating that <italic>AvrMla1</italic> is not genetically fixed in wheat mildew strains.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="publisher-id">plantcell</journal-id><journal-title>The Plant Cell</journal-title><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Physiologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11226189</article-id><article-id pub-id-type="pmc">102246</article-id><article-id pub-id-type="publisher-id">00-0362</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Cell-Autonomous Expression of Barley <italic>Mla1</italic> Confers Race-Specific Resistance to the Powdery Mildew Fungus via a <italic>Rar1</italic>-Independent Signaling Pathway</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Fasong</given-names></name><xref ref-type="fn" rid="fn1">1</xref><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Kurth</surname><given-names>Joachim</given-names></name><xref ref-type="fn" rid="fn1">1</xref><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Wei</surname><given-names>Fusheng</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Elliott</surname><given-names>Candace</given-names></name><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Valè</surname><given-names>Giampiero</given-names></name><xref ref-type="fn" rid="fn2">2</xref><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Yahiaoui</surname><given-names>Nabila</given-names></name><xref ref-type="aff" rid="aff3">c</xref></contrib><contrib contrib-type="author"><name><surname>Keller</surname><given-names>Beat</given-names></name><xref ref-type="aff" rid="aff3">c</xref></contrib><contrib contrib-type="author"><name><surname>Somerville</surname><given-names>Shauna</given-names></name><xref ref-type="aff" rid="aff4">d</xref></contrib><contrib contrib-type="author"><name><surname>Wise</surname><given-names>Roger</given-names></name><xref ref-type="aff" rid="aff2">b</xref><xref ref-type="aff" rid="aff5">e</xref></contrib><contrib contrib-type="author"><name><surname>Schulze-Lefert</surname><given-names>Paul</given-names></name><xref ref-type="fn" rid="fn3">3</xref><xref ref-type="corresp" rid="cor1">4</xref><xref ref-type="aff" rid="aff1">a</xref></contrib></contrib-group><aff id="aff1"><label>a</label>The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom</aff><aff id="aff2"><label>b</label>Interdepartmental Genetics Program and Department of Plant Pathology, Iowa State University, Ames, Iowa 50011-1020</aff><aff id="aff3"><label>c</label>Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland</aff><aff id="aff4"><label>d</label>Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305</aff><aff id="aff5"><label>e</label>Corn Insects and Crop Genetics Research, United States Department of Agriculture, Agricultural Research Service, Iowa State University, Ames, Iowa 50011-1020</aff><author-notes><fn id="fn1"><label>1</label><p>These authors contributed equally to this work.</p></fn><fn id="fn2"><label>2</label><p>Current address: Istituto Sperimentale per la Cerealicoltura, Via S. Protaso 302, I-29017, Fiorenzuola d'Arda (PC), Italy.</p></fn><fn id="fn3"><label>3</label><p>Current address: Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linne-Weg 10, D-50829 Köln, Germany.</p></fn><fn id="cor1"><label>4</label><p>To whom correspondence should be addressed. E-mail <email>schlef@mpiz-koeln.mpg.de</email>; fax 49-221-5062313</p></fn></author-notes><pub-date pub-type="ppub"><month>2</month><year>2001</year></pub-date><volume>13</volume><issue>2</issue><fpage>337</fpage><lpage>350</lpage><history><date date-type="received"><day>25</day><month>8</month><year>2000</year></date><date date-type="accepted"><day>1</day><month>12</month><year>2000</year></date></history><copyright-statement>Copyright © 2001, American Society of Plant Physiologists</copyright-statement><copyright-year>2001</copyright-year><abstract><p>The barley <italic>Mla</italic> locus encodes 28 characterized resistance specificities to the biotrophic fungal pathogen barley powdery mildew. We describe a single-cell transient expression assay using entire cosmid DNAs to pinpoint <italic>Mla1</italic> within the complex 240-kb <italic>Mla</italic> locus. The MLA1 cDNA encodes a 108-kD protein containing an N-terminal coiled-coil structure, a central nucleotide binding domain, and a C-terminal leucine-rich repeat region; it also contains a second short open reading frame at the 5′ end that has a possible regulatory function. Although most <italic>Mla-</italic>encoded resistance specificities require <italic>Rar1</italic> for their function, we used the single-cell expression system to demonstrate that <italic>Mla1</italic> triggers full resistance in the presence of the severely defective <italic>rar1-2</italic> mutant allele. Wheat contains an ortholog of barley <italic>Mla</italic>, designated <italic>TaMla</italic>, that is tightly linked to (0.7 centimorgan) but distinct from a tested resistance specificity at the complex <italic>Pm3</italic> locus to wheat powdery mildew. Thus, the most polymorphic powdery mildew resistance loci in barley and wheat may have evolved in parallel at two closely linked homeoloci. Barley <italic>Mla1</italic> expressed in wheat using the single-cell transformation system failed to trigger a response to any of the wheat powdery mildew <italic>Avr</italic> genes tested, indicating that <italic>AvrMla1</italic> is not genetically fixed in wheat mildew strains.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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